Product Name: Bulk Pack of Positive Control Sera: Ro/SS-A, La/SS-B, Sm, RNP, Scl-70, and Jo-1
ENA Extract is a pool of rabbit and/or bovine origin tissue extracts that will form precipitins with autoantibodies that bind Sm, Ro/SS-A, La/SS-B, nRNP, Scl-70, and Jo-1 when tested by immunodiffusion.
Add 0.25 ml cold distilled water to lyophilized powder (Some assay systems may require more concentrated or more diluted extract.) Some precipitate may form but will not affect the product performance. Do not store diluted antigen. Repeated thawing will result in loss of activity.
After reconstituting, aliquot and store frozen at -20°C.
| Code | Amount | Price |
|---|---|---|
| ENA-1001 | 0.25 ml | $130.80 |
Lyophilized Powder
0.01M Phosphate, 0.13M NaCl, 0.02% Azide
0.02% Azide
Product Name: Ouchterlony gel, 13 well
4°C
| Code | Amount | Price |
|---|---|---|
| ENA-1002 | 5 gels | $118.80 |
0.01M Phosphate, 0.13M NaCl, 0.02% Azide
- Tan EM, Dunkel HG. “Characteristics of a Soluble Nuclear Antigen Precipitation with Sera of Patients with Systemic Lupus Erythematosus.” J of Immunol, Vol: 96, 1966, p 464.
- Tan EM, Rodman GP, Garcia 1, Moroi Y, Fritzler MJ, Peebles C. “Diversity of Antinuclear Antibodies in Progressive Systemic Sclerosis, Anti-Centromere Antibody and its Relationship to CREST Syndrome.” Arthritis Rheum, Vol: 23, 1980, p 617.
Product Name: Ouchterlony gel, 7 well
The ENA Ouchterlony components are useful for the qualitative determination of human autoantibodies in serum to extractable nuclear antigens; Ro/SSA, La/SS-B, Sm, nRNP, Scl-70, and Jo-1. Principle of the Assay: An ENA Ouchterlony assay employs the basic principles of immunodiffusion by incorporating a tissue extract which contains antigens important in many autoimmune diseases. The assay has great utility as: a screening assay, a specific antibody identification assay, and a relative titering assay. The results can be read within 12-24 hours. Scl-70, Ro/SS-A, and Jo-1 antibodies may require 36-48 hours for precipitation. (1)(2)
Product Name: Human anti-Jo-1
Human autoantibody against Jo-1 antigen, buffered with 0.01M phosphate, 0.13 M NaCl, 0.02% NaN3. For use in ENA Ouchterlony kit. Shows only the Jo-1 precipitate band when tested using double immunodiffusion against rabbit thymus extract (RTE-1000). Specificity is verified by identity of precipitation with CDC-AF reference serum. Titer will vary with test system; however, undiluted antibody shows a strong Jo-1 precipitate band.
4°C
| Code | Amount | Price |
|---|---|---|
| HJO-0101 | 0.25 ml | $60.00 |
Lyophilized powder.
- Tan EM, Dunkel HG. “Characteristics of a Soluble Nuclear Antigen Precipitation with Sera of Patients with Systemic Lupus Erythematosus.” J of Immunol, Vol: 96, 1966, p 464.
- Tan EM, Rodman GP, Garcia 1, Moroi Y, Fritzler MJ, Peebles C. “Diversity of Antinuclear Antibodies in Progressive Systemic Sclerosis, Anti-Centromere Antibody and its Relationship to CREST Syndrome.” Arthritis Rheum, Vol: 23, 1980, p 617.
Product Name: Human anti-Scl-70
Human autoantibody against Scl-70 antigen, buffered with 0.01M phosphate, 0.13 M NaCl, 0.02% NaN3. For use in ENA Ouchterlony kit. Shows only the Scl-70 precipitate band when tested using double immunodiffusion against rabbit thymus extract (RTE-1000). Specificity is verified by identity of precipitation with CDC-AF reference serum. Titer will vary with test system; however, undiluted antibody shows a strong Scl-70 precipitate band.
< 0ºC. After reconstituting, aliquot and store frozen at -20°C.
| Code | Amount | Price |
|---|---|---|
| HSC-0101 | 0.25 ml | $60.00 |
Lyophilized powder. Reconstitute with 0.25 ml deionized H2O.
Product Name: Human anti-Sm (Smith)
Human autoantibody against Sm antigen, buffered with 0.01M phosphate, 0.13 M NaCl, 0.02% NaN3. For use in ENA Ouchterlony kit. Shows the Sm precipitate band when tested using double immunodiffusion against rabbit thymus extract (RTE-1000). Specificity is verified by identity of precipitation with CDC-AF reference serum. Anti-RNP precipitant is also detected. Titer will vary with test system; however, undiluted antibody shows a strong Sm precipitate band.
- Tan EM, Dunkel HG. “Characteristics of a Soluble Nuclear Antigen Precipitation with Sera of Patients with Systemic Lupus Erythematosus.” J of Immunol, Vol: 96, 1966, p 464.
- Tan EM, Rodman GP, Garcia 1, Moroi Y, Fritzler MJ, Peebles C. “Diversity of Antinuclear Antibodies in Progressive Systemic Sclerosis, Anti-Centromere Antibody and its Relationship to CREST Syndrome.” Arthritis Rheum, Vol: 23, 1980, p 617.
< 0ºC. After reconstituting, aliquot and store frozen at -20°C.
| Code | Amount | Price |
|---|---|---|
| HSM-0101 | 0.25 ml | $60.00 |
Lyophilized powder. Reconstitute with 0.25 ml deionized H2O.
Product Name: Human anti-RNP
Human autoantibody against RNP antigen, buffered with 0.01M phosphate, 0.13 M NaCl, 0.02% NaN3. For use in ENA Ouchterlony kit. Shows only the RNP precipitate band when tested using double immunodiffusion against rabbit thymus extract (RTE- 1000). Specificity is verified by identity of precipitation with CDCAF reference serum. Titer will vary with test system; however, undiluted antibody shows a strong RNP precipitate band.
- Tan EM, Dunkel HG. “Characteristics of a Soluble Nuclear Antigen Precipitation with Sera of Patients with Systemic Lupus Erythematosus.” J of Immunol, Vol: 96, 1966, p 464.
- Tan EM, Rodman GP, Garcia 1, Moroi Y, Fritzler MJ, Peebles C. “Diversity of Antinuclear Antibodies in Progressive Systemic Sclerosis, Anti-Centromere Antibody and its Relationship to CREST Syndrome.” Arthritis Rheum, Vol: 23, 1980, p 617.
< 0°C. After reconstituting, aliquot and store frozen at -20°C.
| Code | Amount | Price |
|---|---|---|
| HRN-0101 | 0.25 ml | $60.00 |
Lyophilized powder
Product Name: Human anti-SSA
Human autoantibody against SSA antigen, buffered with 0.01M phosphate, 0.13 M NaCl, 0.02% NaN3. For use in ENA Ouchterlony kit. Shows only the SSA precipitate band when tested using double immunodiffusion against bovine spleen extract (BSE-1000). Specificity is verified by identity of precipitation with CDC-AF reference serum. Titer will vary with test system; however, undiluted antibody shows a strong SSA precipitate band.
< 0ºC. After reconstituting, aliquot and store frozen at -20°C.
| Code | Amount | Price |
|---|---|---|
| HSA-0101 | 0.25 ml | $60.00 |
Lyophilized powder. Reconstitute with 0.25 ml deionized H2O.
Product Name: Human anti-La (SSB)
Human autoantibody against SSB antigen, buffered with 0.01M phosphate, 0.13 M NaCl, 0.02% NaN3. For use ENA Ouchterlony kit. Shows only the SSB precipitate band when tested using double immunodiffusion against rabbit thymus extract (RTE-1000). Specificity is verified by identity of precipitation with CDC-AF reference serum. Titer will vary with test system; however, undiluted antibody shows a strong SSB precipitate band.
< 0ºC. After reconstituting, aliquot and store frozen at -20°C.
| Code | Amount | Price |
|---|---|---|
| HSB-0101 | 0.25 ml | $60.00 |
Lyophilized powder. Reconstitute with 0.25 ml deionized H2O